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Journal of Clinical Microbiology, May 2003, p. 2201-2202, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2201-2002.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Influence of Lack of CO2 on Anaerobic Bacteria: a Quick Method To Verify the Absence of CO2

Robbin L. Killam,1 Joan Barenfanger,1* and Mike Cox2

Laboratory Medicine, Memorial Medical Center, Springfield, Illinois,1 Anaerobe Systems, Morgan Hill, California2

Received 19 August 2002/ Returned for modification 29 October 2002/ Accepted 25 January 2003

Since CO2 is carbonic acid gas, it will decrease the pH if added to water. Therefore, a simple test can determine whether CO2 is present in a gas mixture. If the pH of gassed water does not change significantly, then CO2 is not present in the gas.


* Corresponding author. Mailing address: Laboratory Medicine, Memorial Medical Center, 701 N First St., Springfield, IL 62781. Phone: (217) 788-3000. Fax: (217) 788-5577. E-mail: barenfanger.joan{at}mhsil.com.


Journal of Clinical Microbiology, May 2003, p. 2201-2202, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2201-2002.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.