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Journal of Clinical Microbiology, May 2003, p. 2213-2218, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2213-2218.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Polymorphic Region of the rpoB Gene Containing Mycobacterium Species-Specific Sequences and Its Use in Identification of Mycobacteria

Hyeyoung Lee,1 Hye-Eun Bang,2 Gill-Han Bai,3 and Sang-Nae Cho2,4*

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 220-710,1 Department of Microbiology and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752,2 Department of Microbiology, Korean Institute of Tuberculosis, Seoul 137-140,3 The International Vaccine Institute, Seoul 151-600, Republic of Korea4

Received 5 August 2002/ Returned for modification 23 September 2002/ Accepted 23 February 2003

Sequence analysis of a specific region of the mycobacterium rpoB gene in 35 mycobacterial strains representing 26 different mycobacterial species of clinical importance showed that there exists a highly polymorphic region. Based on the sequences of the polymorphic region, the oligonucleotide probes of 14 mycobacterial species with relatively high clinical importance were designed and shown to be specific to their corresponding mycobacterial species by dot blot hybridization. The results showed that the probes designed in this study are highly specific to each mycobacterial species, which suggests that these sequences may be useful for the species identification of mycobacteria.

* Corresponding author. Mailing address: Department of Microbiology, Yonsei University College of Medicine, 134 Shinchon-dong, Seoul 120-752, Republic of Korea. Phone: 822-361-5282. Fax: 822-392-9310. E-mail: raycho{at}yumc.yonsei.ac.kr.

Journal of Clinical Microbiology, May 2003, p. 2213-2218, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2213-2218.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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