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Journal of Clinical Microbiology, June 2003, p. 2440-2443, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2440-2443.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Commercial DNA Extraction Kits for Extraction of Bacterial Genomic DNA from Whole-Blood Samples

K. Smith,1 M. A. Diggle,1 and S. C. Clarke1,2*

Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital,1 Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom2

Received 26 September 2002/ Returned for modification 25 November 2002/ Accepted 27 February 2003

The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction. In addition, it is preferable for these methods to be automated. An example of such a requirement is for the confirmation of meningococcal disease where rapid, sensitive, and specific procedures are required for public health management purposes. Previous studies have shown that whole blood is the preferred method for the isolation of bacterial DNA in meningococcal disease, and in this study, we compare five commercially available kits for the extraction of bacterial genomic DNA from whole-blood samples. These include kits in a 96-well binding plate, 96-well filter plate, and metallic bead formats. The method for all five kits is described, and the sensitivity, specificity, ease of automation, and overall efficiency are determined.

* Corresponding author. Mailing address: Department of Microbiology, Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Balornock Rd., Glasgow G21 3UW, United Kingdom. Phone: 44 141 201 3836. Fax: 44 141 201 3663. E-mail: stuart.clarke{at}northglasgow.scot.nhs.uk.

Journal of Clinical Microbiology, June 2003, p. 2440-2443, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2440-2443.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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