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Journal of Clinical Microbiology, June 2003, p. 2492-2497, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2492-2497.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Human Herpesvirus 8 by Using Single Nucleotide Polymorphisms in Open Reading Frame 26

Tokiomi Endo,1 Toshiyuki Miura,1 Tomohiko Koibuchi,2 Hitomi Nakamura,1 Takashi Takahashi,1 Takashi Odawara,2 Mieko Goto,1 Atsushi Ajisawa,3 Aikichi Iwamoto,1,2 and Tetsuya Nakamura2*

Division of Infectious Diseases, Advanced Clinical Research Center,1 Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo,2 Tokyo Metropolitan Komagome Hospital, Tokyo, Japan3

Received 15 October 2002/ Returned for modification 22 December 2002/ Accepted 22 February 2003

Human herpesvirus 8 (HHV-8) can be classified into distinct subtypes on the basis of sequence polymorphisms in several open reading frames (ORFs). We analyzed the subtypes of HHV-8 in 59 human immunodeficiency virus-infected Japanese patients by using polymorphisms in ORF26 and found that over two-thirds of the HHV-8 isolates fell into major subtype A. We also found that single nucleotide polymorphisms (SNPs) at nucleotide positions 1032 (C-to-A substitution) and 1055 (G-to-T substitution) in HHV-8 ORF26 were correlated with increased susceptibility to Kaposi's sarcoma, compared to the results obtained with HHV-8 with wild-type nucleotides at these positions (P = 0.0106). This observation suggests that molecular heterogeneity of the HHV-8 genome affects the biological properties of HHV-8, resulting in different clinical phenotypes of HHV-8 infection. Since sensitive PCR of ORF26 allowed us to analyze the SNPs by using peripheral blood from HHV-8-infected patients, the ORF26 SNPs will be a potent tool for investigating the pathogenesis of HHV-8 infection.

* Corresponding author. Mailing address: Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-3-5449-5338. Fax: 81-3-5449-5427. E-mail: tnakamur{at}ims.u-tokyo.ac.jp.

Journal of Clinical Microbiology, June 2003, p. 2492-2497, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2492-2497.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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