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Journal of Clinical Microbiology, June 2003, p. 2662-2664, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2662-2664.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Washing and Swabbing Procedures for Collecting Genital Fluids To Assess Cervicovaginal Shedding of Herpes Simplex Virus Type 2 DNA

Angélique Ndjoyi-Mbiguino,1 Francis Ozouaki,2 Jérôme Legoff,3 François-Xavier Mbopi-Kéou,4 Ali Si-Mohamed,3 Isabelle Ndombi Onas,5 Evelyne Avoune,1 and Laurent Bélec3,6*

Laboratoire de Virologie, Département de Microbiologie, Université des Sciences de la Santé,1 Maternité Joséphine Bongo,2 Service de Gynécologie et Obstétrique, Centre Hospitalier de Libreville, Libreville, Gabon,5 Laboratoire de Virologie, Hôpital Européen Georges Pompidou, and Université Pierre et Marie Curie (Paris VI),3 Equipe Transmission Muqueuse du VIH et Immunité Locale, Unité INSERM 430, Institut Biologique des Cordeliers, Faculté de Médecine Broussais-Hôtel Dieu, Paris, France,6 Department of Oral Medicine, Eastman Institute, UCL, University of London, London, United Kingdom4

Received 17 June 2002/ Returned for modification 20 September 2002/ Accepted 28 February 2003

Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Européen Georges Pompidou, 20 Rue Leblanc, 75908 Paris Cedex 15, France. Phone: 331-56-09-39-59. Fax: 331-56-09-24-47. E-mail: laurent.belec{at}egp.ap-hop-paris.fr.

Journal of Clinical Microbiology, June 2003, p. 2662-2664, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2662-2664.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Legoff, J., Bouhlal, H., Gresenguet, G., Weiss, H., Khonde, N., Hocini, H., Desire, N., Si-Mohamed, A., de Dieu Longo, J., Chemin, C., Frost, E., Pepin, J., Malkin, J.-E., Mayaud, P., Belec, L. (2006). Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV. J. Clin. Microbiol. 44: 423-432 [Abstract] [Full Text]  
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