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Journal of Clinical Microbiology, June 2003, p. 2669-2671, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2669-2671.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli

Claudia Toma,1* Yan Lu,1 Naomi Higa,1 Noboru Nakasone,1 Isabel Chinen,2 Ariela Baschkier,2 Marta Rivas,2 and Masaaki Iwanaga1

Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan,1 Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas, ANLIS-"Dr. Carlos G. Malbrán," 1281 Buenos Aires, Argentina2

Received 16 December 2002/ Accepted 25 February 2003

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.

* Corresponding author. Mailing address: Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan. Phone: 81-98-895-1124. Fax: 81-98-895-1408. E-mail: k950417{at}med.u-ryukyu.ac.jp.

Journal of Clinical Microbiology, June 2003, p. 2669-2671, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2669-2671.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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