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Journal of Clinical Microbiology, June 2003, p. 2694-2696, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2694-2696.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Stability of Repetitive-Sequence PCR Patterns with Respect to Culture Age and Subculture Frequency

Hyunseok Peter Kang and W. Michael Dunne*

Division of Laboratory Medicine, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110

Received 23 December 2002/ Returned for modification 12 February 2003/ Accepted 7 March 2003

To examine the stability of repetitive-sequence (rep) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted from the cultures after 24, 48, and 72 h of incubation at 35°C. In addition, the same species were subcultured to fresh blood plates daily and DNA was extracted from the cultures after growth of 5, 10, and 15 subcultures, respectively. rep PCR analysis demonstrated that all rep PCR fingerprints from a single species were identical.

* Corresponding author. Mailing address: Department of Pathology and Immunology, Washington University School of Medicine, Campus Box 8118, 660 S. Euclid Ave., St. Louis, MO 63110. Phone: (314) 362-1547. Fax: (314) 362-1461. E-mail: dunne{at}labmed.wustl.edu.

Journal of Clinical Microbiology, June 2003, p. 2694-2696, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2694-2696.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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