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Journal of Clinical Microbiology, June 2003, p. 2716-2722, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2716-2722.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Bovine Non-O157 Shiga Toxin 2-Containing Escherichia coli Isolates Commonly Possess stx2-EDL933 and/or stx2vhb Subtypes

Kim N. Brett,1,2 Michael A. Hornitzky,1 Karl A. Bettelheim,3 Mark J. Walker,2 and Steven P. Djordjevic1*

Elizabeth Macarthur Agricultural Institute, Camden, New South Wales, Australia 2570,1 Department of Biological Sciences, University of Wollongong, New South Wales, Australia 2522,2 Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 30523

Received 28 May 2002/ Accepted 18 December 2002

stx2 genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx2-EDL933 (103 of 127; 81.1%) either in combination with stx2vhb (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx2-EDL933 and stx2vhb; 2 isolates contained stx2vhb only. Although 7 of 11 (63.6%) human O113:H21 isolates associated with diarrhea possessed stx2-EDL933, the remaining 4 isolates possessed a combination of stx2-EDL933 and stx2vhb. Three of the four were from separate sporadic cases of HUS, and one was from an unknown source.

* Corresponding author. Mailing address: NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Private Mail Bag, 8 Camden, NSW 2570, Australia. Phone: 61-246-406426. Fax: 61-246-406384. E-mail: steve.djordjevic{at}agric.nsw.gov.au.

Journal of Clinical Microbiology, June 2003, p. 2716-2722, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2716-2722.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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