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Journal of Clinical Microbiology, June 2003, p. 2723-2726, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2723-2726.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of the Manila Family of Mycobacterium tuberculosis

James T. Douglas,1* Lishi Qian,1 Jaime C. Montoya,2 James M. Musser,3 Jan D. A. Van Embden,4 Dick Van Soolingen,4 and Kristin Kremer4

Department of Microbiology, University of Hawaii, Honolulu, Hawaii,1 Research Institute for Tropical Medicine and Philippine General Hospital, University of the Philippines, Manila, Republic of the Philippines,2 Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana,3 National Institute of Public Health and the Environment, Bilthoven, The Netherlands4

Received 7 October 2002/ Returned for modification 5 December 2002/ Accepted 10 March 2003

Forty-eight Mycobacterium tuberculosis strains were obtained from patients living in metropolitan Manila, Republic of the Philippines. Three molecular typing methods, IS6110 restriction fragment length polymorphism, spoligotyping, and DNA sequencing of the oxyR, gyrA, and katG loci, established that these strains have restricted diversity and are members of a related genetic group of organisms. Comparison of the DNA fingerprint patterns with those in international databases confirmed the uniqueness of this group of isolates, which we designate the Manila family of M. tuberculosis.

* Corresponding author. Mailing address: University of Hawaii at Manoa, Department of Microbiology, 2538 The Mall (Snyder Hall 207), Honolulu, HI 96822. Phone: (808) 956-4639. Fax: (808) 956-5350. E-mail: jdouglas{at}hawaii.edu.

Journal of Clinical Microbiology, June 2003, p. 2723-2726, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2723-2726.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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