This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quarleri, J. F.
Right arrow Articles by Oubiña, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quarleri, J. F.
Right arrow Articles by Oubiña, J. R.
Right arrowPubmed/NCBI databases
*Protein

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2003, p. 2727-2733, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2727-2733.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

In Vitro Detection of Dissimilar Amounts of Hepatitis C Virus (HCV) Subtype-Specific RNA Genomes in Mixes Prepared from Sera of Persons Infected with a Single HCV Genotype

Jorge F. Quarleri,1 María V. Bussy,1 Verónica L. Mathet,1 Vanesa Ruiz,1 Rubén Iácono,1 Ling Lu,2 Betty H. Robertson,2 and José R. Oubiña1*

Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina,1 Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 15 November 2002/ Returned for modification 8 January 2003/ Accepted 25 January 2003

The level of in vitro detection of viral genomes in mixes with two different hepatitis C virus (HCV) subtypes was investigated by artificially mixing previously measured subtype-specific HCV RNA genomes. The RNAs in these mixtures were reverse transcribed and then PCR amplified by using two sets of primers corresponding to the 5' untranslated region and digested with endonucleases to analyze the restriction fragment length polymorphism patterns. This approach facilitated detection of a wider range of type-specific HCV genomes than originally described, beyond equimolar concentrations of contributing HCV subtypes. Moreover, by using computerized image analysis, this study also demonstrated that the true contribution of each virus type—and consequently of mixed infections—may be underestimated when only visual observation is carried out. These results may be useful for comparing data obtained from this and other currently used methodologies.

* Corresponding author. Mailing address: Hepatitis Laboratory, Microbiology Department, School of Medicine, University of Buenos Aires, Paraguay 2155 - Piso 11, (1121) Buenos Aires, Argentina. Phone: (5411) 5950-9500/99. Fax: (5411) 4508-3705. E-mail address: joubina{at}fmed.uba.ar.

Journal of Clinical Microbiology, June 2003, p. 2727-2733, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2727-2733.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Antonishyn, N. A., Ast, V. M., McDonald, R. R., Chaudhary, R. K., Lin, L., Andonov, A. P., Horsman, G. B. (2005). Rapid Genotyping of Hepatitis C Virus by Primer-Specific Extension Analysis. J. Clin. Microbiol. 43: 5158-5163 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»