This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Studentsov, Y. Y.
Right arrow Articles by Burk, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Studentsov, Y. Y.
Right arrow Articles by Burk, R. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2003, p. 2827-2834, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.2827-2834.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Polymer-Based Enzyme-Linked Immunosorbent Assay Using Human Papillomavirus Type 16 (HPV16) Virus-Like Particles Detects HPV16 Clade-Specific Serologic Responses

Yevgeniy Y. Studentsov,1 Gloria Y. F. Ho,2 Morgan A. Marks,1 Robert Bierman,3 and Robert D. Burk1,2,4,5*

Department of Microbiology and Immunology,1 Department of Epidemiology and Social Medicine,2 Department of Pediatrics and,4 Department of Obstetrics & Gynecology and Women's Health,5 Albert Einstein College of Medicine, Bronx, New York,5 Rutgers University Student Health Service, New Brunswick, New Jersey3

Received 17 January 2003/ Returned for modification 18 March 2003/ Accepted 27 March 2003

Human papillomavirus type 16 (HPV16) virus-like particles (VLP) were used as antigen in a polymer enzyme-linked immunosorbent assay (ELISA) to measure antibodies to HPV capsid proteins. Serum samples from 575 college women, previously tested for the presence of cervicovaginal HPV DNA, were analyzed. The prevalences of anti-HPV16 VLP antibodies at baseline were 14.1% for immunoglobulin G (IgG) and 6.4% for IgA. The seroprevalences of IgG in women with cervicovaginal HPV16, HPV16-related types, and other HPV types were 55, 33, and 19%, respectively (P < 0.001), compared to the prevalence in women without an HPV infection (10%). HPV VLP IgA seropositivity was associated with high HPV16 VLP IgG optical density values. The seropositivity of IgG antibodies was independently associated with infection with HPV16 or HPV16-related types, increased number of lifetime male partners for vaginal sex, having sex with men >=5 years older, history of abnormal PAP smear, older age, and living separately from parents. Use of HPV16 VLP polymer ELISA detects clade-specific responses and suggests an HPV16 VLP vaccine may have broader protection that initially anticipated.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, Cancer Research Center, Ullmann Bldg., Rm. 515, Bronx, NY 10461. Phone: (718) 430-3720. Fax: (718) 430-8975. E-mail: burk{at}aecom.yu.edu.

Journal of Clinical Microbiology, July 2003, p. 2827-2834, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.2827-2834.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marais, D. J., Constant, D., Allan, B., Carrara, H., Hoffman, M., Shapiro, S., Morroni, C., Williamson, A.-L. (2008). Cervical Human Papillomavirus (HPV) Infection and HPV Type 16 Antibodies in South African Women. J. Clin. Microbiol. 46: 732-739 [Abstract] [Full Text]  
  • Clifford, G. M., Shin, H.-R., Oh, J.-K., Waterboer, T., Ju, Y.-H., Vaccarella, S., Quint, W., Pawlita, M., Franceschi, S. (2007). Serologic Response to Oncogenic Human Papillomavirus Types in Male and Female University Students in Busan, South Korea. Cancer Epidemiol. Biomarkers Prev. 16: 1874-1879 [Abstract] [Full Text]  
  • Shields, T. S., Brinton, L. A., Burk, R. D., Wang, S. S., Weinstein, S. J., Ziegler, R. G., Studentsov, Y. Y., McAdams, M., Schiffman, M. (2004). A Case-Control Study of Risk Factors for Invasive Cervical Cancer among U.S. Women Exposed to Oncogenic Types of Human Papillomavirus. Cancer Epidemiol. Biomarkers Prev. 13: 1574-1582 [Abstract] [Full Text]  
  • Ho, G. Y. F., Studentsov, Y. Y., Bierman, R., Burk, R. D. (2004). Natural History of Human Papillomavirus Type 16 Virus-Like Particle Antibodies in Young Women. Cancer Epidemiol. Biomarkers Prev. 13: 110-116 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»