This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Makanera, A.
Right arrow Articles by Manai, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Makanera, A.
Right arrow Articles by Manai, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2003, p. 2940-2945, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.2940-2945.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology and Characterization of Plasmid-Encoded ß-Lactamases Produced by Tunisian Clinical Isolates of Salmonella enterica Serotype Mbandaka Resistant to Broad-Spectrum Cephalosporins

Abdoulaye Makanera,1 Guillaume Arlet,2,3* Valérie Gautier,3 and Mohamed Manai1*

Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Tunis, Département de Biologie, Université de Tunis El Manar, 2092 Tunis, Tunisia,1 Service de Bactériologie-Hygiène, Hôpital Tenon, AP-HP,2 Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine,Paris, France3

Received 9 October 2002/ Returned for modification 21 January 2003/ Accepted 13 March 2003

We studied 31 clinical isolates of Salmonella enterica serotype Mbandaka resistant to broad-spectrum cephalosporins and recovered in Tunisia over a 5-year period. The transferability of this resistance was demonstrated by conjugation experiments. Thirty of the 31 isolates were positive in the double-disk synergy test. By isoelectric focusing analysis, all of the isolates were found to produce a band of ß-lactamase activity with a pI of 5.9. Three of these isolates produced an additional band with a pI of 7.6. PCR and DNA sequencing identified these ß-lactamases as TEM-4 and SHV-2a, respectively. The remaining isolate, highly resistant to ceftazidime but susceptible to cefepime, produced a ß-lactamase that focused at pI 7.8. No synergy was detected by the double-disk synergy test. Sequence analysis of the bla gene amplified by PCR showed that the plasmid-mediated AmpC-type enzyme was ACC-1a. Fingerprinting analysis by repetitive-element PCR and enterobacterial repeat intergenic consensus-PCR suggested that 29 of the 31 Salmonella serotype Mbandaka isolates belonged to the same clonal population.

* Corresponding author. Mailing address for Guillaume Arlet: Service de Bactériologie-Hygiène, Hôpital Tenon, 4 Rue de la Chine, 75970 Paris Cedex 20, France. Phone: 33 1 56 01 70 18. Fax: 33 1 56 01 61 08. E-mail: guillaume.arlet{at}tnn.ap-hop-paris.fr. Mailing address for Mohamed Manai: Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Tunis, Département de Biologie, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia. Phone: 216 71 872 600. Fax: 216 71 885 480. E-mail: mohamedmanai{at}yahoo.fr.

Journal of Clinical Microbiology, July 2003, p. 2940-2945, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.2940-2945.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Papagiannitsis, C. C., Tzouvelekis, L. S., Tzelepi, E., Miriagou, V. (2007). Plasmid-Encoded ACC-4, an Extended-Spectrum Cephalosporinase Variant from Escherichia coli. Antimicrob. Agents Chemother. 51: 3763-3767 [Abstract] [Full Text]  
  • Doloy, A., Verdet, C., Gautier, V., Decre, D., Ronco, E., Hammami, A., Philippon, A., Arlet, G. (2006). Genetic Environment of Acquired blaACC-1 {beta}-Lactamase Gene in Enterobacteriaceae Isolates. Antimicrob. Agents Chemother. 50: 4177-4181 [Abstract] [Full Text]  
  • Ruppe, E., Bidet, P., Verdet, C., Arlet, G., Bingen, E. (2006). First Detection of the Ambler Class C 1 AmpC {beta}-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test. J. Clin. Microbiol. 44: 4204-4207 [Abstract] [Full Text]  
  • Chouchani, C., Berlemont, R., Masmoudi, A., Galleni, M., Frere, J.-M., Belhadj, O., Ben-Mahrez, K. (2006). A Novel Extended-Spectrum TEM-Type {beta}-Lactamase, TEM-138, from Salmonella enterica Serovar Infantis.. Antimicrob. Agents Chemother. 50: 3183-3185 [Abstract] [Full Text]  
  • Bidet, P., Burghoffer, B., Gautier, V., Brahimi, N., Mariani-Kurkdjian, P., El-Ghoneimi, A., Bingen, E., Arlet, G. (2005). In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae. Antimicrob. Agents Chemother. 49: 3562-3565 [Abstract] [Full Text]  
  • Bouallegue-Godet, O., Salem, Y. B., Fabre, L., Demartin, M., Grimont, P. A. D., Mzoughi, R., Weill, F.-X. (2005). Nosocomial Outbreak Caused by Salmonella enterica Serotype Livingstone Producing CTX-M-27 Extended-Spectrum {beta}-Lactamase in a Neonatal Unit in Sousse, Tunisia. J. Clin. Microbiol. 43: 1037-1044 [Abstract] [Full Text]  
  • Miro, E., Mirelis, B., Navarro, F., Matas, L., Gimenez, M., Rabaza, C. (2005). Escherichia coli Producing an ACC-1 Class C {beta}-Lactamase Isolated in Barcelona, Spain. Antimicrob. Agents Chemother. 49: 866-867 [Full Text]  
  • Weill, F.-X., Lailler, R., Praud, K., Kerouanton, A., Fabre, L., Brisabois, A., Grimont, P. A. D., Cloeckaert, A. (2004). Emergence of Extended-Spectrum-{beta}-Lactamase (CTX-M-9)-Producing Multiresistant Strains of Salmonella enterica Serotype Virchow in Poultry and Humans in France. J. Clin. Microbiol. 42: 5767-5773 [Abstract] [Full Text]  
  • Kruger, T., Szabo, D., Keddy, K. H., Deeley, K., Marsh, J. W., Hujer, A. M., Bonomo, R. A., Paterson, D. L. (2004). Infections with Nontyphoidal Salmonella Species Producing TEM-63 or a Novel TEM Enzyme, TEM-131, in South Africa. Antimicrob. Agents Chemother. 48: 4263-4270 [Abstract] [Full Text]  
  • Weill, F.-X., Demartin, M., Fabre, L., Grimont, P. A. D. (2004). Extended-Spectrum-{beta}-Lactamase (TEM-52)-Producing Strains of Salmonella enterica of Various Serotypes Isolated in France. J. Clin. Microbiol. 42: 3359-3362 [Abstract] [Full Text]  
  • Weill, F.-X., Demartin, M., Tande, D., Espie, E., Rakotoarivony, I., Grimont, P. A. D. (2004). SHV-12-Like Extended-Spectrum-{beta}-Lactamase-Producing Strains of Salmonella enterica Serotypes Babelsberg and Enteritidis Isolated in France among Infants Adopted from Mali. J. Clin. Microbiol. 42: 2432-2437 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»