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Journal of Clinical Microbiology, July 2003, p. 3051-3055, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3051-3055.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Major Outbreak of Toxic Shock-Like Syndrome Caused by Streptococcus mitis

Hong-Zhou Lu,1,2 Xin-Hua Weng,2 Bai Zhu,3 Haijing Li,1 You-Kuan Yin,2 Yong-Xin Zhang,2 David W. Haas,1,4 and Yi-Wei Tang1,5*

Departments of Medicine,1 Microbiology and Immunology,4 Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232,5 Department of Infectious Diseases, Fudan University Huashan Hospital, Shanghai,2 Haian People's Hospital, Jiangsu, China3

Received 24 January 2003/ Returned for modification 27 February 2003/ Accepted 7 April 2003

Severe illness caused by viridans streptococci rarely occurs in immunocompetent hosts. Between December 1990 and May 1991, thousands of patients in the YangZi River Delta area of Jiangsu Province, China, suffered from scarlet fever-like pharyngitis. Fewer cases occurred in subsequent years with the same seasonality. Approximately half of the cases developed complications characteristic of streptococcal toxic shock-like syndrome (TSLS). Throat cultures yielded predominant growth of alpha-hemolytic streptococci. All cases admitted to Haian People's Hospital were investigated. Clinical specimens were collected, medical records were reviewed, and bacterial isolates were identified phenotypically and analyzed by 16S rRNA gene sequencing and pulsed-field gel electrophoresis (PFGE). Proteins were purified from culture supernatants by extraction, ammonium sulfate precipitation, and fast-protein liquid chromatography. Biological activities of protein components were determined by subcutaneous inoculation into rabbits. A total of 178 cases of non-beta-hemolytic streptococcal scarlet fever-like pharyngitis were studied. In 88 (79.3%) of 111 patients, oropharyngeal swab cultures grew morphologically identical alpha-hemolytic streptococci. A protein in culture supernatants was pyrogenic in rabbits, was mitogenic for splenocytes, and enhanced rabbit susceptibility to endotoxin challenge. The N-terminal amino acid sequence of this 34-kDa protein showed no homology with known Streptococcus pyrogenic exotoxins. The organism was identified as Streptococcus mitis based on biochemical and 16S rRNA sequence analyses. Representative outbreak isolates from 1990 to 1995 displayed identical PFGE patterns. This TSLS outbreak in southeastern China was caused by a toxigenic clone of S. mitis. An apparently novel toxin may explain the unusual virulence of this organism.

* Corresponding author. Mailing address: A3310 MCN, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN 37232-2605. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: yiwei.tang{at}vanderbilt.edu.

Journal of Clinical Microbiology, July 2003, p. 3051-3055, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3051-3055.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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