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Journal of Clinical Microbiology, July 2003, p. 3312-3316, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3312-3316.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Selective Medium for Isolation of Burkholderia pseudomallei

K. Howard* and T. J. J. Inglis

Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Australia

Received 23 August 2002/ Returned for modification 8 October 2002/ Accepted 18 December 2002

Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas aeruginosa were used to determine the selectivity and sensitivity of BPSA. BPSA was more inhibitory to P. aeruginosa and B. cepacia and should make recognition of Burkholderia species easier due to distinctive colony morphology. BPSA also inhibited Enterococcus, Escherichia, Staphylococcus, and Streptococcus. These results indicate that BPSA is a potential replacement for ASA.

* Corresponding author. Mailing address: Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research (PathCentre), Locked Bag 2009, Nedlands, WA 6909, Australia. Phone: 618 9346 3640. Fax: 618 9381 7139. E-mail: khoward{at}cyllene.uwa.edu.au.

Journal of Clinical Microbiology, July 2003, p. 3312-3316, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3312-3316.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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