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Journal of Clinical Microbiology, July 2003, p. 3317-3319, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3317-3319.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

First Isolation and Identification of Rickettsia conorii from Ticks Collected in the Region of Fokida in Central Greece

Anna Psaroulaki,* Ioanna Spyridaki, Alexandros Ioannidis, Thomas Babalis, Achilleas Gikas, and Yannis Tselentis

Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, Collaborating Center of the World Health Organization, University of Heraklion, 71409 Crete, Greece

Received 23 October 2002/ Returned for modification 15 December 2002/ Accepted 12 February 2003

Three different spotted-fever group rickettsiae—Rickettsia conorii, R. massiliae, and R. rhipicephali—were detected and identified by PCR-restriction fragment length polymorphism analysis in Rhipicephalus ticks collected from domestic animals in the Fokida region of Greece, where a high seroprevalence of antibodies to R. conorii was previously demonstrated. The infection rate of ticks was 1.6%. Moreover, R. conorii was isolated from one Rhipicephalus sanguineus tick.


* Corresponding author. Mailing address: Laboratory of Clinical Bacteriology, Parasitology, and Geographical Medicine, Faculty of Medicine, University of Crete, P.O. Box 1393, TK 71409 Heraklion of Crete, Greece. Phone: 30 2810-394743. Fax: 30 2810-394740. E-mail: annapsa{at}med.uoc.gr.


Journal of Clinical Microbiology, July 2003, p. 3317-3319, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3317-3319.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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