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Journal of Clinical Microbiology, July 2003, p. 3323-3326, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3323-3326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Staphylococcus aureus with DNA Array Technology

Willem B. van Leeuwen,1* Corinne Jay,2 Susan Snijders,1 Nathalia Durin,2 Bruno Lacroix,2 Henry A. Verbrugh,1 Mark C. Enright,3 Alain Troesch,2 and Alex van Belkum1

Department of Medical Microbiology & Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands,1 bioMérieux, Marcy l'Etoile, France,2 University of Bath, Bath, United Kingdom3

Received 15 January 2003/ Returned for modification 12 March 2003/ Accepted 11 April 2003

A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.

* Corresponding author. Mailing address: Erasmus MC, Department of Medical Microbiology & Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31-10-4633668. Fax: 31-10-4633875. E-mail: vanleeuwen{at}erasmusmc.nl.

Journal of Clinical Microbiology, July 2003, p. 3323-3326, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3323-3326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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