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Journal of Clinical Microbiology, July 2003, p. 3351-3353, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3351-3353.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Use of Recombinant Em18 and Affinity-Purified Em18 for Serological Differentiation of Alveolar Echinococcosis from Cystic Echinococcosis and Other Parasitic Infections

Ning Xiao,^1,2 Wulamu Mamuti,^1,3 Hiroshi Yamasaki,¹ Yasuhito Sako,¹ Minoru Nakao,¹ Kazuhiro Nakaya,⁴ Bruno Gottstein,⁵ Peter M. Schantz,⁶ Marshall W. Lightowlers,⁷ Philip S. Craig,⁸ and Akira Ito^1*

Department of Parasitology,¹ Animal Laboratory for Medical Research,Asahikawa Medical College, Asahikawa, Japan,⁴ Sichuan Institute of Parasitic DiseasesChengdu,² Department of Parasitology, Xinjiang Medical University, Urumqi, China,³ Institute of Parasitology, University of Berne, Berne, Switzerland,⁵ Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,⁶ Veterinary Clinical Center, University of Melbourne, Melbourne, Australia,⁷ Division of Biological Sciences, School of Environment and Life Sciences, University of Salford, Great Manchester, United Kingdom⁸

Received 10 February 2003/ Returned for modification 23 March 2003/ Accepted 14 April 2003

To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.

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* Corresponding author. Mailing address: Department of Parasitology, Asahikawa Medical College, Midorigaoka-Higashi 2-1-1-1, Asahikawa, 078-8510, Japan. Phone: 81-166-68-2420. Fax: 81-166-68-2429. akiraito{at}asahikawa-med.ac.jp.

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Journal of Clinical Microbiology, July 2003, p. 3351-3353, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3351-3353.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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