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Journal of Clinical Microbiology, July 2003, p. 3372-3375, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3372-3375.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a Duplex PCR Assay for Detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in Pig Feces

Tom La, Nyree D. Phillips, and David J. Hampson*

School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia

Received 3 February 2003/ Returned for modification 23 March 2003/ Accepted 25 April 2003

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilosicoli.

* Corresponding author. Mailing address: School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia. Phone: 61 8 9360 2287. Fax: 61 8 9310 4144. E-mail: D.Hampson{at}murdoch.edu.au.

Journal of Clinical Microbiology, July 2003, p. 3372-3375, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3372-3375.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Feberwee, A., Hampson, D. J., Phillips, N. D., La, T., van der Heijden, H. M. J. F., Wellenberg, G. J., Dwars, R. M., Landman, W. J. M. (2008). Identification of Brachyspira hyodysenteriae and Other Pathogenic Brachyspira Species in Chickens from Laying Flocks with Diarrhea or Reduced Production or Both. J. Clin. Microbiol. 46: 593-600 [Abstract] [Full Text]  
  • Corona-Barrera, E., Smith, D. G.E., La, T., Hampson, D. J., Thomson, J. R. (2004). Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces. J Med Microbiol 53: 301-307 [Abstract] [Full Text]  
  • Jamshidian, M., La, T., Phillips, N. D., Hampson, D. J. (2004). Brachyspira pilosicoli colonization in experimentally infected mice can be facilitated by dietary manipulation. J Med Microbiol 53: 313-318 [Abstract] [Full Text]  


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