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Journal of Clinical Microbiology, July 2003, p. 3410-3412, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3410-3412.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Quality Control Limits for Fluconazole Disk Susceptibility Tests on Mueller-Hinton Agar with Glucose and Methylene Blue

A. Barry,1* J. Bille,2 S. Brown,1 D. Ellis,3 J. Meis,4 M. Pfaller,5 R. Rennie,6 M. Rinaldi,7 T. Rogers,8 and M. Traczewski1

The Clinical Microbiology Institute, Wilsonville, Oregon,1 Institute of Microbiology, University Hospital, Lausanne, Switzerland,2 Women and Children's Hospital, North Adelaide, South Australia, Australia,3 Canisius-Wilhelmina Hospital, Center for Infectious Diseases, Nijmegen, The Netherlands,4 University of Iowa, Iowa City, Iowa,5 University of Alberta Hospital, Edmonton, Alberta, Canada,6 University of Texas Health Science Center, San Antonio, Texas,7 Imperial College, Hammersmith, London, England8

Received 9 April 2003/ Returned for modification 30 March 2003/ Accepted 19 April 2003

An international collaborative study was performed in order to propose quality control limits for fluconazole disk diffusion susceptibility tests on Mueller-Hinton agar with 2% glucose and 0.5 µg of methylene blue per ml. The supplements may be added before autoclaving the agar, or Mueller-Hinton agar plates may be flooded with a glucose-methylene blue solution. Replicate tests on both types of agar plates generated data to propose zone size limits for tests of Candida parapsilosis ATCC 22019 (22 to 33 mm), C. tropicalis ATCC 750 (26 to 37 mm), and C. albicans ATCC 90028 (28 to 39 mm). C. krusei ATCC 6258 was not useful for this purpose.

* Corresponding author. Mailing address: The Clinical Microbiology Institute, 9725 SW Commerce Circle, Suite A1, Wilsonville, OR 97070. Phone: (503) 682-3232. Fax: (503) 682-2065. E-mail: cmi{at}hevanet.com.

Journal of Clinical Microbiology, July 2003, p. 3410-3412, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3410-3412.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pfaller, M. A., Diekema, D. J., Sheehan, D. J. (2006). Interpretive Breakpoints for Fluconazole and Candida Revisited: a Blueprint for the Future of Antifungal Susceptibility Testing. Clin. Microbiol. Rev. 19: 435-447 [Abstract] [Full Text]  
  • Pfaller, M. A., Barry, A., Bille, J., Brown, S., Ellis, D., Meis, J. F., Rennie, R., Rinaldi, M., Rogers, T., Traczewski, M. (2004). Quality Control Limits for Voriconazole Disk Susceptibility Tests on Mueller-Hinton Agar with Glucose and Methylene Blue. J. Clin. Microbiol. 42: 1716-1718 [Abstract] [Full Text]  
  • Pfaller, M. A., Boyken, L., Messer, S. A., Hollis, R. J., Diekema, D. J. (2004). Stability of Mueller-Hinton Agar Supplemented with Glucose and Methylene Blue for Disk Diffusion Testing of Fluconazole and Voriconazole. J. Clin. Microbiol. 42: 1288-1289 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., Diekema, D. J. (2004). Evaluation of the NCCLS M44-P Disk Diffusion Method for Determining Susceptibilities of 276 Clinical Isolates of Cryptococcus neoformans to Fluconazole. J. Clin. Microbiol. 42: 380-383 [Abstract] [Full Text]  


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