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Journal of Clinical Microbiology, July 2003, p. 3419-3422, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3419-3422.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Fungal Burden in Experimental Paracoccidioidomycosis by Using the Fluorescent Dye Blankophor

Angela Satie Nishikaku and Eva Burger*

Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil

Received 27 January 2003/ Returned for modification 16 March 2003/ Accepted 10 April 2003

The fungal load in organs and blood of susceptible and resistant mice infected with Paracoccidioides brasiliensis was quantitated by using the optical brightener Blankophor and compared with CFU counts. Fluorescent staining of fungal cells proved to be a quick and easy procedure, suitable for evaluation of paracoccidioidomycotic infection.

* Corresponding author. Mailing address: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, 1730, Cidade Universit ria, 05508-900, São Paulo, SP, Brazil. Phone: 55-11-3091-7378. Fax: 55-11-3091-7224. E-mail: evburger{at}icb.usp.br.

Journal of Clinical Microbiology, July 2003, p. 3419-3422, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3419-3422.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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