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Journal of Clinical Microbiology, August 2003, p. 3494-3498, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3494-3498.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Epidemiological Survey of Babesia Species in Japan Performed with Specimens from Ticks Collected from Dogs and Detection of New Babesia DNA Closely Related to Babesia odocoilei and Babesia divergens DNA

Hisashi Inokuma,1* Yukako Yoshizaki,1 Yojiro Shimada,2 Yoshimi Sakata,3 Masaru Okuda,1 and Takafumi Onishi1

Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515,1 Nippon Zenyaku Kogyo Co., Ltd, Koriyama, Fukushima 963-0196,2 Merial Japan Ltd., Tokyo 100-0014, Japan3

Received 10 January 2003/ Returned for modification 9 March 2003/ Accepted 12 May 2003

Detection and analysis of Babesia species from ticks recovered from dogs in Japan were attempted by PCR and nucleotide sequence analysis based on the 18S rRNA gene, respectively. A total of 1,136 ticks were examined for Babesia DNA by 18S rRNA-based PCR and nucleotide sequencing. Partial sequences of Babesia canis vogeli DNA were detected from six ticks in Aomori, Nara, Hiroshima, Oita, and Okinawa Prefectures; and Babesia gibsoni Asia-1 DNA was also detected in four ticks in Osaka, Hiroshima, Miyazaki, and Okinawa Prefectures. Unique sequences of 1,678 bp were also obtained from Ixodes ovatus ticks in Akita and Fukui Prefectures. The sequences were similar to those of Babesia odocoilei (97.7%) and Babesia divergens (97.6%). This is the first report of the detection of DNA belonging to this group in Japan.

* Corresponding author. Mailing address: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. Phone and fax: 81-83-933-5895. E-mail: inokuma{at}yamaguchi-u.ac.jp.

Journal of Clinical Microbiology, August 2003, p. 3494-3498, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3494-3498.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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