This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ajayi, T.
Right arrow Articles by Wiener-Kronish, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ajayi, T.
Right arrow Articles by Wiener-Kronish, J. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2003, p. 3526-3531, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3526-3531.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Single-Nucleotide-Polymorphism Mapping of the Pseudomonas aeruginosa Type III Secretion Toxins for Development of a Diagnostic Multiplex PCR System

Temitayo Ajayi,1,2 Leonard R. Allmond,3 Teiji Sawa,3 and Jeanine P. Wiener-Kronish1,2,4*

Department of Anesthesia and Perioperative Care,3 Cardiovascular Research Institute,1 Division of Pulmonary and Critical Care Medicine,2 Department of Medicine, University of California, San Francisco, San Francisco, California 941434

Received 4 February 2003/ Returned for modification 22 April 2003/ Accepted 1 May 2003

We mapped the coding single nucleotide polymorphisms in four toxin genes—exoS, exoT, exoU, and exoY—of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and specificity in this small sample size. This assay appears to be promising for the rapid and accurate genotyping of the presence of these genes in clinical strains of P. aeruginosa.

* Corresponding author. Mailing address: Anesthesia Department, 513 Parnassus Ave., S-261, University of California, San Francisco, CA 94143-0542. Phone: (415) 476-8968. Fax: (415) 476-8841. E-mail: WienerKJ{at}anesthesia.ucsf.edu.

Journal of Clinical Microbiology, August 2003, p. 3526-3531, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3526-3531.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, H.-H., Huang, S.-p., Teng, H.-C., Ji, D.-D., Chen, Y.-S., Chen, Y.-L. (2006). Presence of the exoU Gene of Pseudomonas aeruginosa Is Correlated with Cytotoxicity in MDCK Cells but Not with Colonization in BALB/c Mice. J. Clin. Microbiol. 44: 4596-4597 [Abstract] [Full Text]  
  • Winstanley, C., Kaye, S. B, Neal, T. J, Chilton, H. J, Miksch, S., Hart, C A., and the Microbiology Ophthalmic Group, (2005). Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis. J Med Microbiol 54: 519-526 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»