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Journal of Clinical Microbiology, August 2003, p. 3661-3667, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3661-3667.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens

Elizabeth B. Kauffman,* Susan A. Jones, Alan P. Dupuis II, Kiet A. Ngo, Kristen A. Bernard, and Laura D. Kramer

The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, Slingerlands, New York 12159

Received 22 January 2003/ Returned for modification 20 April 2003/ Accepted 10 May 2003

The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions of the world have made it essential to develop efficient, sensitive, and rapid protocols for virus surveillance. Laboratory testing is the backbone of any surveillance program. Protocols to detect the presence of WNV have been refined since 1999 for sensitivity, speed, efficiency, and specificity. This paper presents the protocols currently used by the New York State Department of Health to handle vertebrate and mosquito specimens that have been submitted for WNV testing to the Arbovirus Laboratories of the Wadsworth Center.

* Corresponding author. Mailing address: The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Rd., Slingerlands, NY 12159. Phone: (518) 869-4585. Fax: (518) 869-4530. E-mail: Ebk01{at}health.state.ny.us.

Journal of Clinical Microbiology, August 2003, p. 3661-3667, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3661-3667.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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