This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kourova, N.
Right arrow Articles by Guiso, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kourova, N.
Right arrow Articles by Guiso, N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2003, p. 3706-3711, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3706-3711.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of the Bordetella pertussis and Bordetella parapertussis Isolates Circulating in Saint Petersburg between 1998 and 2000 with Russian Vaccine Strains

Natacha Kourova,1 Valérie Caro,2 Christian Weber,2 Sabine Thiberge,2 Raisa Chuprinina,3 Galina Tseneva,1 and Nicole Guiso2*

Bacterial Respiratory Infections Laboratory, Pasteur Institute of Epidemiology and Microbiology, 197101 Saint Petersburg, Russia,1 Unité des Bordetella, Institut Pasteur, 75724 Paris Cedex 15, France,2 Bacterial Vaccines Control and Standardization Laboratory, L. A. Tarassevich State Control and Standardization Institute, 121002 Moscow, Russia3

Received 10 February 2003/ Returned for modification 7 April 2003/ Accepted 14 May 2003

We analyzed the Bordetella pertussis and Bordetella parapertussis isolates circulating in Saint Petersburg that were collected between 1998 and 2000 and compared them with isolates collected 40 years ago and Russian vaccine strains. The analysis involved serotyping, pulsed-field gel electrophoresis of chromosomal DNA after digestion with XbaI and SpeI, and sequencing of the ptxS1 and prn genes, which encode the S1 subunit of the pertussis toxin and the major adhesin pertactin, respectively. The Russian isolates were classified in five of the six pulsed-field gel electrophoresis groups identified in other European countries. The B. pertussis isolates currently circulating in Saint Petersburg differed from the Russian whole-cell vaccine strains and the isolates collected in the prevaccine era. However, their repartition in the major pulsed-field gel electrophoresis groups was slightly different from that of isolates collected in countries that have had a high level of vaccine coverage for a long time, probably because the level of vaccine coverage in Saint Petersburg has increased only recently, after decreasing until the early 1990s. Most of the B. parapertussis isolates studied were similar to those circulating in France. However, some variants were observed, perhaps because B. parapertussis infections are more common in children in this area.

* Corresponding author. Mailing address: Unité des Bordetella. Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 8334. Fax: 33 1 40 61 35 33. E-mail: nguiso{at}pasteur.fr.

Journal of Clinical Microbiology, August 2003, p. 3706-3711, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3706-3711.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Litt, D. J., Neal, S. E., Fry, N. K. (2009). Changes in Genetic Diversity of the Bordetella pertussis Population in the United Kingdom between 1920 and 2006 Reflect Vaccination Coverage and Emergence of a Single Dominant Clonal Type. J. Clin. Microbiol. 47: 680-688 [Abstract] [Full Text]  
  • Andre, P., Caro, V., Njamkepo, E., Wendelboe, A. M., Van Rie, A., Guiso, N. (2008). Comparison of Serological and Real-Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007. J. Clin. Microbiol. 46: 1672-1677 [Abstract] [Full Text]  
  • Borisova, O., Kombarova, S. Y., Zakharova, N. S., van Gent, M., Aleshkin, V. A., Mazurova, I., Mooi, F. R. (2007). Antigenic Divergence between Bordetella pertussis Clinical Isolates from Moscow, Russia, and Vaccine Strains. CVI 14: 234-238 [Abstract] [Full Text]  
  • Kodama, A., Kamachi, K., Horiuchi, Y., Konda, T., Arakawa, Y. (2004). Antigenic Divergence Suggested by Correlation between Antigenic Variation and Pulsed-Field Gel Electrophoresis Profiles of Bordetella pertussis Isolates in Japan. J. Clin. Microbiol. 42: 5453-5457 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»