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Journal of Clinical Microbiology, August 2003, p. 3864-3867, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3864-3867.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Improved Molecular Detection of Dengue Virus Serotype 1 Variants

Jean-Marc Reynes,1* Sivuth Ong,1 Channa Mey,1 Chantha Ngan,2 Stefan Hoyer,3 and Amadou A. Sall1

Institut Pasteur du Cambodge,1 National Dengue Control Programme, Ministry of Health,2 World Health Organization, Phnom Penh, Cambodia3

Received 10 September 2002/ Returned for modification 26 February 2003/ Accepted 23 May 2003

The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. Vance-Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.

* Corresponding author. Mailing address: Jean-Marc Reynes, Institut Pasteur du Cambodge, 5 boulevard Monivong, BP 983, Phnom Penh, Cambodia. Phone: (855) 12 802 981. Fax: (855) 23 725 606. E-mail: jmreynes{at}pasteur-kh.org or jmreynes{at}bigpond.com.kh.

Journal of Clinical Microbiology, August 2003, p. 3864-3867, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3864-3867.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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