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Journal of Clinical Microbiology, August 2003, p. 3890-3892, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3890-3892.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) in Blood with the EVIGENE MRSA Detection Kit

Katrina Levi* and Kevin J. Towner

Molecular Diagnostics and Typing Unit, Department of Microbiology, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom

Received 31 March 2003/ Returned for modification 5 May 2003/ Accepted 15 May 2003

A total of 200 blood cultures containing putative staphylococci were analyzed by a commercial gene probe hybridization assay (EVIGENE; Statens Serum Institut, Copenhagen, Denmark), and 18 were identified as methicillin-resistant Staphylococcus aureus (MRSA) positive. Of these, 17 were positive by PCR and 16 were positive by culture. Detailed analysis of the discrepant results showed that the EVIGENE kit allowed specific identification of MRSA in blood cultures without any of the drawbacks associated with PCR.

* Corresponding author. Mailing address: Molecular Diagnostics and Typing Unit, Department of Microbiology, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom. Phone: (44) 115-970 9163. Fax: (44) 115-942 2190. E-mail: katrina.levi{at}mail.qmcuh-tr.trent.nhs.uk.

Journal of Clinical Microbiology, August 2003, p. 3890-3892, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3890-3892.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Brown, D. F. J., Edwards, D. I., Hawkey, P. M., Morrison, D., Ridgway, G. L., Towner, K. J., Wren, M. W. D., on behalf of the Joint Working Party of the Britis, (2005). Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 56: 1000-1018 [Abstract] [Full Text]  
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