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Journal of Clinical Microbiology, August 2003, p. 3901-3904, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3901-3904.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

Béatrice Nicollier-Jamot, Valérie Pico, Pierre Pothier, and Evelyne Kohli*

Microbiologie Médicale et Moléculaire, Facultés de Médecine et Pharmacie, Université de Bourgogne, 21033 Dijon cedex, France

Received 18 March 2002/ Returned for modification 20 July 2002/ Accepted 24 April 2003

Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1,078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.

* Corresponding author. Mailing address: Laboratoire de Microbiologie Médicale et Moléculaire, Facultés de Médecine et Pharmacie, Université de Bourgogne, 7 boulevard Jeanne d'Arc, 21033 Dijon Cedex, France. Phone: 33 3 80 29 34 37. Fax: 33 3 80 29 36 04. E-mail: Evelyne.Kohli{at}u-bourgogne.fr.

Journal of Clinical Microbiology, August 2003, p. 3901-3904, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3901-3904.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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