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Journal of Clinical Microbiology, August 2003, p. 3939-3941, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3939-3941.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Dipsticks for Rapid Diagnosis of Cholera Caused by Vibrio cholerae O1 and O139 from Rectal Swabs

N. A. Bhuiyan,1 Firdausi Qadri,1 A. S. G. Faruque,1 M. A Malek,1 M. A. Salam,1 Farida Nato,2 J. M. Fournier,2 S. Chanteau,3 David A. Sack,1 and G. Balakrish Nair1*

Laboratory and Clinical Sciences Divisions, International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B), Dhaka 1212, Bangladesh,1 Institut Pasteur, Paris, France,2 CERMES, Niamey, Niger3

Received 13 March 2003/ Returned for modification 9 May 2003/ Accepted 20 May 2003

We evaluated the recently developed dipsticks for the rapid detection of Vibrio cholerae serotypes O1 and O139 from rectal swabs of hospitalized diarrheal patients after enrichment for 4 h in alkaline peptone water. The sensitivity and specificity of the dipsticks were above 92 and 91%, respectively. The dipsticks represent the first rapid test which has been successfully used to diagnose cholera from rectal swabs, and this would immensely improve surveillance for cholera, especially in remote settings.

* Corresponding author. Mailing address: Laboratory Sciences Division, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR, B), Mohakhali, Dhaka-1212, Bangladesh. Phone: 880-2-9886464. Fax: 880-2-8823116. E-mail: gbnair{at}icddrb.org.

Journal of Clinical Microbiology, August 2003, p. 3939-3941, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3939-3941.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tuteja, U., Kumar, S., Shukla, J., Kingston, J., Batra, H. V. (2007). Simultaneous direct detection of toxigenic and non-toxigenic Vibrio cholerae from rectal swabs and environmental samples by sandwich ELISA. J Med Microbiol 56: 1340-1345 [Abstract] [Full Text]  


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