This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miller, R. A. Articles by Reimschuessel, R. Search for Related Content PubMed PubMed Citation Articles by Miller, R. A. Articles by Reimschuessel, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2003, p. 4318-4323, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4318-4323.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Antimicrobial Susceptibility Testing of Aquatic Bacteria: Quality Control Disk Diffusion Ranges for Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 at 22 and 28°C

R. A. Miller,^1* R. D. Walker,¹ A. Baya,² K. Clemens,³ M. Coles,⁴ J. P. Hawke,⁵ B. E. Henricson,⁶ H. M. Hsu,⁷ J. J. Mathers,⁸ J. L. Oaks,⁹ M. Papapetropoulou,¹⁰ and R. Reimschuessel¹

Division of Animal and Food Microbiology, Center for Veterinary Medicine, Food and Drug Administration, Laurel, Maryland 20708,¹ Animal Health Diagnostic Laboratory, Maryland Department of Agriculture, College Park, Maryland 20740,² Idaho Fish Health Center, U.S. Fish and Wildlife Service, Ahsahka, Idaho 83520,³ Aquatic Diagnostic Services, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada C1A 4P3,⁴ School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803,⁵ Virginia Department of Agriculture and Consumer Services, Warrenton, Virginia 20186,⁶ Wisconsin Veterinary Diagnostic Laboratory, University of Wisconsin, Madison, Wisconsin 53705,⁷ Animal Health Division, Alpharma, Chicago Heights, Illinois 60411,⁸ Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, Washington 99164,⁹ Laboratory of Public Health, University of Patras, 26500 Rio Patras, Greece,¹⁰

Received 4 April 2003/ Returned for modification 23 May 2003/ Accepted 11 June 2003

Quality control (QC) ranges for disk diffusion susceptibility testing of aquatic bacterial isolates were proposed as a result of a multilaboratory study conducted according to procedures established by the National Committee for Clinical Laboratory Standards (NCCLS). Ranges were proposed for Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 at 22 and 28°C for nine different antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, gentamicin, oxolinic acid, oxytetracycline, ormetoprim-sulfadimethoxine, and trimethoprim-sulfamethoxazole). All tests were conducted on standard Mueller-Hinton agar. With 95% of all data points fitting within the proposed QC ranges, the results from this study comply with NCCLS guidelines and have been accepted by the NCCLS Subcommittee for Veterinary Antimicrobial Susceptibility Testing. These QC guidelines will permit greater accuracy in interpreting results and, for the first time, the ability to reliably compare susceptibility test data between aquatic animal disease diagnostic laboratories.

---

* Corresponding author. Mailing address: U.S. Food and Drug Administration, Center for Veterinary Medicine, HFV530, 8401 Muirkirk Rd., Laurel, MD 20708. Phone: (301) 827-7902. Fax: (301) 827-8250. E-mail: RMiller1{at}cvm.fda.gov.

---

Journal of Clinical Microbiology, September 2003, p. 4318-4323, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4318-4323.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Welch, T. J., Evenhuis, J., White, D. G., McDermott, P. F., Harbottle, H., Miller, R. A., Griffin, M., Wise, D. (2009). IncA/C Plasmid-Mediated Florfenicol Resistance in the Catfish Pathogen Edwardsiella ictaluri. Antimicrob. Agents Chemother. 53: 845-846 [Full Text]  
• McIntosh, D., Cunningham, M., Ji, B., Fekete, F. A., Parry, E. M., Clark, S. E., Zalinger, Z. B., Gilg, I. C., Danner, G. R., Johnson, K. A., Beattie, M., Ritchie, R. (2008). Transferable, multiple antibiotic and mercury resistance in Atlantic Canadian isolates of Aeromonas salmonicida subsp. salmonicida is associated with carriage of an IncA/C plasmid similar to the Salmonella enterica plasmid pSN254. J Antimicrob Chemother 61: 1221-1228 [Abstract] [Full Text]