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Journal of Clinical Microbiology, September 2003, p. 4408-4410, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4408-4410.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Chemically Defined Media for Growth of Haemophilus influenzae Strains

Hannah N. Coleman,{dagger} Dayle A. Daines, Justin Jarisch, and Arnold L. Smith*

Seattle Biomedical Research Institute, Seattle, Washington 98109

Received 16 February 2003/ Returned for modification 27 April 2003/ Accepted 9 June 2003

A chemically defined medium that supports the growth of both encapsulated and nontypeable Haemophilus influenzae strains in broth to densities that are >= 109 CFU/ml or on agar plates is described. The mean generation time of a panel of clinical isolates was comparable to that in rich, chemically undefined media (brain-heart infusion broth supplemented with heme and ß-NAD).

* Corresponding author. Mailing address: Seattle Biomedical Research Institute, 4 Nickerson St., Suite 200, Seattle, WA 98109. Phone: (206) 284-8846. Fax: (206) 284-0313. E-mail: arnold.smith{at}sbri.org.

{dagger} Present address: Department of Molecular Microbiology & Immunology, School of Medicine, University of Missouri—Columbia, Columbia, MO 65212.

Journal of Clinical Microbiology, September 2003, p. 4408-4410, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4408-4410.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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