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Journal of Clinical Microbiology, September 2003, p. 4424-4427, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4424-4427.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Population Dynamics of Persistent Staphylococcus aureus Isolated from the Airways of Cystic Fibrosis Patients during a 6-Year Prospective Study

Barbara C. Kahl,1* Angelika Duebbers,2 Gabriele Lubritz,1 Johannes Haeberle,2 Hans G. Koch,2 Barbara Ritzerfeld,1 Marion Reilly,1 Erik Harms,2 Richard A. Proctor,3 Mathias Herrmann,4 and Georg Peters1

Institute of Medical Microbiology,1 Department of Pediatrics, University of Muenster Hospital and Clinics, Muenster,2 Institute of Medical Microbiology and Hygiene, University of Saarland Hospital and Clinics, Homburg, Germany,4 Department of Medicine and Medical Microbiology/Immunology, University of Wisconsin Medical School, Madison, Wisconsin3

Received 10 March 2003/ Returned for modification 20 April 2003/ Accepted 27 June 2003

Molecular typing of normal (n = 456) and small-colony-variant (SCV; n = 239) Staphylococcus aureus isolates cultured from the airways of 52 of 72 cystic fibrosis (CF) patients (72.2%) during a 6-year prospective study revealed a median long-term persistence of 37 months (range, 6 to 70). SCV persisted longer in the airways than the normal S. aureus (statistically not significant). Pulsed-field gel electrophoresis identified six prevalent clonal lineages, which were cultured from more than one patient (3 to 12 patients), and 39 individual clones, which were isolated only from single patients. The SCV phenotype was not restricted to a distinct clonal lineage but occurred in many different clones. Most patients (33 of 52, 63.46%) harbored single clones. This study provides a basis for improved understanding of S. aureus colonization and infection dynamics in CF patients.

* Corresponding author. Mailing address: Inst. f. Med. Mikrobiologie, Domagkstr. 10, 48149 Muenster, Germany. Phone: 049-251-8355381. Fax. 049-251-8355350. E-mail: kahl{at}uni-muenster.de.

Journal of Clinical Microbiology, September 2003, p. 4424-4427, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4424-4427.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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