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Journal of Clinical Microbiology, September 2003, p. 4431-4434, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4431-4434.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Highly Sensitive PCR Assay for Routine Diagnosis of African Swine Fever Virus in Clinical Samples

M. Agüero,1* J. Fernández,1 L. Romero,1 C. Sánchez Mascaraque,1 M. Arias,1 and J. M. Sánchez-Vizcaíno2

Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid,1 Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain2

Received 28 March 2003/ Returned for modification 26 May 2003/ Accepted 22 June 2003

This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.

* Corresponding author. Mailing address: Centro de Investigación en Sanidad Animal (CISA-INIA), Ctra. Algete-El Casar, Valdeolmos, 28130 Madrid, Spain. Phone: 34 91 6202300. Fax: 34 91 6202247. E-mail: aguero{at}inia.es.

Journal of Clinical Microbiology, September 2003, p. 4431-4434, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4431-4434.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Zsak, L., Borca, M. V., Risatti, G. R., Zsak, A., French, R. A., Lu, Z., Kutish, G. F., Neilan, J. G., Callahan, J. D., Nelson, W. M., Rock, D. L. (2005). Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay. J. Clin. Microbiol. 43: 112-119 [Abstract] [Full Text]  


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