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Journal of Clinical Microbiology, September 2003, p. 4454-4456, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4454-4456.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Direct Detection of Multidrug-Resistant Mycobacterium tuberculosis in Clinical Specimens in Low- and High-Incidence Countries by Line Probe Assay

Isik Somuncu Johansen,1* Bettina Lundgren,2 Anaida Sosnovskaja,3 and Vibeke Østergaard Thomsen1

International Reference Laboratory of Mycobacteriology, Statens Serum Institut,1 Department of Clinical Microbiology, Hvidovre University Hospital, Copenhagen, Denmark,2 National Tuberculosis Reference Laboratory, Vilnius, Lithuania3

Received 31 October 2002/ Returned for modification 9 March 2003/ Accepted 8 June 2003

The INNO-LiPA Rif.TB assay is designed for the detection of rpoB gene mutations causing rifampin resistance in isolates. We applied the method directly to 60 Lithuanian and Danish clinical specimens to detect rifampin resistance rapidly. Results were obtained in 78.3% of clinical specimens, and all were concordant with those obtained by BACTEC 460. The assay could have major impact on the management of multidrug-resistant tuberculosis.

* Corresponding author. Mailing address: International Reference Laboratory of Mycobacteriology, 5 Artillerivej, 2300 Copenhagen, Denmark. Phone: 4532683703. Fax: 4532683871. E-mail: isj{at}ssi.dk.

Journal of Clinical Microbiology, September 2003, p. 4454-4456, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4454-4456.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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