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Journal of Clinical Microbiology, September 2003, p. 4457-4459, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4457-4459.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Difference in FKS1 Gene Sequences between Serotypes A and D of Cryptococcus neoformans

Reiko Tanaka,* Yumi Imanishi,{dagger} and Kazuko Nishimura

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan

Received 15 April 2003/ Returned for modification 21 April 2003/ Accepted 11 June 2003

We compared sequences of the glucan synthase (FKS1) gene in serotypes A and D of Cryptococcus neoformans. Four introns were present in serotype D but not serotype A. PCR with primers that flank these introns permits simple differentiation of serotypes A and D.

* Corresponding author. Mailing address: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan. Phone: 81-43-226-2788. Fax: 81-43-226-2486. E-mail: moru{at}faculty.chiba-u.jp.

{dagger} Present address: NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu-shi, Chiba 292-0818, Japan.

Journal of Clinical Microbiology, September 2003, p. 4457-4459, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4457-4459.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Enache-Angoulvant, A., Chandenier, J., Symoens, F., Lacube, P., Bolognini, J., Douchet, C., Poirot, J. L., Hennequin, C. (2007). Molecular Identification of Cryptococcus neoformans Serotypes. J. Clin. Microbiol. 45: 1261-1265 [Abstract] [Full Text]  
  • Stajich, J. E., Dietrich, F. S. (2006). Evidence of mRNA-Mediated Intron Loss in the Human-Pathogenic Fungus Cryptococcus neoformans. Eukaryot Cell 5: 789-793 [Abstract] [Full Text]  


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