This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Bost, S.
Right arrow Articles by Mainil, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Bost, S.
Right arrow Articles by Mainil, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2003, p. 4480-4482, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4480-4482.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli

Sigrid Van Bost,1* Etienne Jacquemin,1 Eric Oswald,2 and Jacques Mainil1

University of Liège, Faculty of Veterinary Medicine, Laboratory of Bacteriology, Sart-Tilman B43a, B-4000 Liège, Belgium,1 Institut National de la Recherche Agronomique, Unité de Microbiologie Moléculaire, 31076 Toulouse Cedex, France2

Received 5 May 2003/ Returned for modification 13 June 2003/ Accepted 3 July 2003

Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species.

* Corresponding author. Mailing address: Université de Liège, Faculté de Médecine Vétérinaire, Microbiologie des Denrées Alimentaires B43bis, 20 Bd. de Colonster, B-4000 Liège, Belgium. Phone: 32 4 366 40 14. Fax: 32 4 366 40 16. E-mail: svanbost{at}ulg.ac.be.

Journal of Clinical Microbiology, September 2003, p. 4480-4482, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4480-4482.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»