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Journal of Clinical Microbiology, January 2004, p. 193-197, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.193-197.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Dipstick Assay for Detection of Leishmania-Specific Canine Antibodies

Henk D. F. H. Schallig,1* Luís Cardoso,2 Marieke Hommers,1 Nel Kroon,1 Guus Belling,3 Manuela Rodrigues,2 Saul J. Semião-Santos,4 and Hans Vetter3

KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute), KIT Biomedical Research,1 Department of Microbiology, Academic Medical Centre, Amsterdam, The Netherlands,3 Departamento de Higiene e Sanidade, ICETA, Universidade de Trás-os-Montes e Alto Douro, Vila Real,2 DepBio/CDI-LEISH, Universidade de Évora, Évora, Portugal4

Received 7 July 2003/ Returned for modification 19 September 2003/ Accepted 5 October 2003

A dipstick assay, based on Leishmania infantum antigen, for the rapid detection of Leishmania-specific antibodies in canine serum samples was developed and evaluated. After determination of optimal dipstick test conditions, test performance was compared with two existing serological tests, i.e., the direct agglutination test (DAT) and the fast agglutination screening test (FAST). In the present study the dipstick test had a sensitivity of 99.2% and a specificity of 87.9%. The DAT had a sensitivity of 97.7% and a specificity of 95.2%, whereas the FAST had also a sensitivity of 97.7% and a specificity of 93.0%. High degrees of agreement were observed between the dipstick test and DAT (93.7%; {kappa} value, 0.86), between the dipstick test and FAST (91.8%; {kappa} value, 0.82), and between the DAT and FAST (95.2%; {kappa} value, 0.90). The high sensitivity and ease of performance make the dipstick test very suitable for surveillance surveys.

* Corresponding author. Mailing address: KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute), KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. Phone: 31 20 5665447. Fax: 31 20 6971841. E-mail: H.Schallig{at}kit.nl.

Journal of Clinical Microbiology, January 2004, p. 193-197, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.193-197.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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