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Journal of Clinical Microbiology, January 2004, p. 347-350, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.347-350.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Severe Acute Respiratory Syndrome Coronavirus in Blood of Infected Patients

Lisa F. P. Ng,1 Michelle Wong,2 Susie Koh,2 Eng-Eong Ooi,3 King-Fai Tang,3 Hoe-Nam Leong,4 Ai-Ee Ling,5 Lora V. Agathe,1 Jenny Tan,1 Edison T. Liu,1 Ee-Chee Ren,1,6 Lee-Ching Ng,2 and Martin L. Hibberd1*

Genome Institute of Singapore,1 DSO National Laboratories,2 Environmental Health Institute, National Environment Agency,3 Tan Tock Seng Hospital,4 Department of Pathology, Singapore General Hospital and,5 Department of Microbiology, National University of Singapore, Singapore, Republic of Singapore6

Received 3 July 2003/ Returned for modification 9 September 2003/ Accepted 25 September 2003

Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.

* Corresponding author. Mailing address: Genome Institute of Singapore, 60 Biopolis St., Genome, #02-01, Singapore 138672, Republic of Singapore. Phone: 65-647-88087. Fax: 65-647-89004. E-mail: hibberdml{at}gis.a-star.edu.sg.

Journal of Clinical Microbiology, January 2004, p. 347-350, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.347-350.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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