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Journal of Clinical Microbiology, January 2004, p. 359-361, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.359-361.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of an Immunochromatographic Test with Recombinant EMA-2 for the Rapid Detection of Antibodies against Babesia equi in Horses

Xiaohong Huang,1,2 Xuenan Xuan,1 Longshan Xu,2 Shoufa Zhang,3 Naoaki Yokoyama,1 Naoyoshi Suzuki,1 and Ikuo Igarashi1*

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan,1 Fujian Center for Disease Control and Prevention, Fuzhou 350001,2 Department of Veterinary Medicine, Yanbian University, Longjing, Jilin Province 133400, China3

Received 3 March 2003/ Returned for modification 18 August 2003/ Accepted 13 September 2003

An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and uninfected horses. The agreement with enzyme-linked immunosorbent assay results was 96.7% in the detection of field sera. The results suggest that BeICT is rapid, simple, reliable, and suitable for use to detect B. equi infection in the field.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5641. Fax: 81-155-49-5643. E-mail: igarcpmi{at}obihiro.ac.jp.

Journal of Clinical Microbiology, January 2004, p. 359-361, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.359-361.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, X., Xuan, X., Verdida, R. A., Zhang, S., Yokoyama, N., Xu, L., Igarashi, I. (2006). Immunochromatographic Test for Simultaneous Serodiagnosis of Babesia caballi and B. equi Infections in Horses.. CVI 13: 553-555 [Abstract] [Full Text]  


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