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Journal of Clinical Microbiology, January 2004, p. 390-392, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.390-392.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Mutations Including IS6110 Insertion in the Gene Encoding the MPB64 Protein of Capilia TB-Negative Mycobacterium tuberculosis Isolates

Kazue Hirano,1* Akio Aono,2 Mitsuyoshi Takahashi,1 and Chiyoji Abe1

Department of Basic Research, Research Institute of Tuberculosis,1 Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association, Kiyose-shi, Tokyo 204-8533, Japan2

Received 19 June 2003/ Returned for modification 7 August 2003/ Accepted 5 October 2003

A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.

* Corresponding author. Mailing address: Department of Basic Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose-shi, Tokyo 204-8533, Japan. Phone: 81-424-93-5762. Fax: 81-424-92-4600. E-mail: hirano{at}jata.or.jp.

Journal of Clinical Microbiology, January 2004, p. 390-392, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.390-392.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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