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Journal of Clinical Microbiology, January 2004, p. 474-477, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.474-477.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Transfer of a Mycobacterium tuberculosis Genotyping Method, Spoligotyping, from a Reverse Line-Blot Hybridization, Membrane-Based Assay to the Luminex Multianalyte Profiling System

Lauren S. Cowan,* Lois Diem, Mary Catherine Brake, and Jack T. Crawford

Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 15 September 2003/ Returned for modification 10 October 2003/ Accepted 21 October 2003

Spoligotyping using Luminex technology was shown to be a highly reproducible method suitable for high-throughput analysis. Spoligotyping of 48 isolates using the traditional membrane-based assay and the Luminex assay yielded concordant results for all isolates. The Luminex platform provides greater flexibility and cost effectiveness than the membrane-based assay.

* Corresponding author. Mailing address: Mailstop F08, CDC, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-1481. Fax: (404) 639-1287. E-mail: LCowan{at}cdc.gov.

Journal of Clinical Microbiology, January 2004, p. 474-477, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.474-477.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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