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Journal of Clinical Microbiology, October 2004, p. 4799-4802, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4799-4802.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

New Method for Laboratory Detection of AmpC ß-Lactamases in Escherichia coli and Klebsiella pneumoniae

K. Nasim,1 S. Elsayed,1,2 J. D. D. Pitout,1,2 J. Conly,1,3 D. L. Church,1,2,3 and D. B. Gregson1,2,3*

Department of Pathology and Laboratory Medicine,1 Department of Medicine, University of Calgary,3 Calgary Laboratory Services, Calgary, Alberta, Canada2

Received 6 November 2003/ Returned for modification 12 March 2004/ Accepted 26 April 2004

A new cefoxitin-agar medium (CAM)-based assay was compared to the previously published modified three-dimensional (M3D) assay for the detection of AmpC production in Escherichia coli and Klebsiella pneumoniae. Clinical isolates of cefoxitin-resistant E. coli (n = 5) and K. pneumoniae (n = 7) and multiple control strains with and without AmpC enzymes were tested by both methods. The CAM method with 4 µg of cefoxitin/ml was equivalent to the M3D method for detecting AmpC production in E. coli and K. pneumoniae. This new method is easier to perform and interpret and allows for testing of multiple isolates on a single plate.

* Corresponding author. Mailing address: Calgary Laboratory Services, #9, 3535 Research Rd., NW, Calgary, Alberta, Canada T2L 2K8. Phone: (403) 770-3309. Fax: (403) 770-3347. E-mail: dan.gregson{at}cls.ab.ca.

Journal of Clinical Microbiology, October 2004, p. 4799-4802, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4799-4802.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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