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Journal of Clinical Microbiology, October 2004, p. 4818-4820, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4818-4820.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Diagnostic Value of Reverse Transcription-PCR of Amniotic Fluid for Prenatal Diagnosis of Congenital Rubella Infection in Pregnant Women with Confirmed Primary Rubella Infection

Muriel Macé,1 Denis Cointe,1 Caroline Six,2 Daniel Levy-Bruhl,2 Isabelle Parent du Châtelet,2 Didier Ingrand,1 and Liliane Grangeot-Keros1*

Service de Microbiologie-Immunologie biologique, Hôpital Antoine Béclère, Clamart,1 Institut de Veille Sanitaire, Saint Maurice, France2

Received 18 February 2004/ Returned for modification 15 April 2004/ Accepted 1 July 2004

The diagnostic value of RT-PCR of amniotic fluid (AF) for prenatal diagnosis of congenital rubella virus infection in 45 pregnant women with confirmed primary infection was assessed. Specificity of RT-PCR was 100% and sensitivity ranged between 83 and 95%.


* Corresponding author. Mailing address: Service de Microbiologie-Immunologie biologique, Hôpital Antoine Béclère, 92141 Clamart cedex, France. Phone: (33)-1-45-37-42-98. Fax: (33)-1-46-32-67-96. E-mail: liliane.keros{at}abc.ap-hop-paris.fr


Journal of Clinical Microbiology, October 2004, p. 4818-4820, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4818-4820.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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