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Journal of Clinical Microbiology, October 2004, p. 4835-4837, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4835-4837.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Catheter-Related Bloodstream Infections by the Differential-Time-to-Positivity Method and Gram Stain-Acridine Orange Leukocyte Cytospin Test in Neutropenic Patients after Hematopoietic Stem Cell Transplantation

R. Krause,1* H. W. Auner,2 G. Gorkiewicz,3 A. Wölfler,2 F. Daxboeck,4 W. Linkesch,2 G. J. Krejs,1 C. Wenisch,1 and E. C. Reisinger5

Division of Infectious Diseases,1 Division of Hematology,2 Department of Internal Medicine, and Institute of Hygiene, Medical University of Graz, Graz,3 Clinical Institute for Hygiene and Medical Microbiology, Division of Hospital Hygiene, University of Vienna, Vienna, Austria,4 Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, University of Rostock, Rostock, Germany5

Received 26 April 2004/ Returned for modification 4 June 2004/ Accepted 5 July 2004

For febrile neutropenic patients who received hematopoietic stem cell transplantation, the Gram stain-acridine orange leukocyte cytospin (AOLC) test and the differential-time-to-positivity method (DTP) were performed. As a diagnostic tool for catheter-related bloodstream infections in these patients, the Gram stain-AOLC test has a lower sensitivity than does the DTP method but acceptable positive and negative predictive values.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, A-8036 Graz, Austria. Phone: 43-316-385-2274. Fax: 43-316-385-3062. E-mail: robert.krause{at}meduni-graz.at.

Journal of Clinical Microbiology, October 2004, p. 4835-4837, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4835-4837.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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