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Journal of Clinical Microbiology, October 2004, p. 4850-4852, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4850-4852.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multiplex Detection of Mutations in Clinical Isolates of Rifampin-Resistant Mycobacterium tuberculosis by Short Oligonucleotide Ligation Assay on DNA Chips

Jiao-Yu Deng,1,2 Xian-En Zhang,1* Hong-Bing Lu,1 Qiang Liu,1 Zhi-Ping Zhang,1 Ya-Feng Zhou,1 Wei-Hong Xie,1 and Zhi-Jun Fu3

Wuhan Institute of Virology,1 Wuhan Institute of Hydrobiology, Chinese Academy of Sciences,2 Wuhan Tuberculosis Prevention and Cure Institute, Wuhan, People's Republic of China3

Received 23 March 2004/ Returned for modification 28 April 2004/ Accepted 8 June 2004

A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing.

* Corresponding author. Mailing address: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. Phone: 86 10 58881058. Fax: 86 27 87199492. E-mail: zhangxe{at}mail.most.gov.cn.

Journal of Clinical Microbiology, October 2004, p. 4850-4852, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4850-4852.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miller, M. B., Tang, Y.-W. (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22: 611-633 [Abstract] [Full Text]  
  • Aragon, L. M., Navarro, F., Heiser, V., Garrigo, M., Espanol, M., Coll, P. (2006). Rapid detection of specific gene mutations associated with isoniazid or rifampicin resistance in Mycobacterium tuberculosis clinical isolates using non-fluorescent low-density DNA microarrays. J Antimicrob Chemother 57: 825-831 [Abstract] [Full Text]  


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