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Journal of Clinical Microbiology, October 2004, p. 4859-4862, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4859-4862.2004

Comparative Analysis of the Schleicher and Schuell IsoCode Stix DNA Isolation Device and the Qiagen QIAamp DNA Mini Kit

Susan R. Coyne,1 Philip D. Craw,1 David A. Norwood,1 and Melanie P. Ulrich2*

Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick,1 Goldbelt Raven, LLC, Frederick, Maryland2

Received 22 April 2004/ Returned for modification 7 June 2004/ Accepted 19 June 2004

Efficient, rapid, and reproducible procedures for isolating high-quality DNA before PCR gene amplification are essential for the diagnostic and molecular identification of pathogenic bacteria. This study evaluated the Qiagen QIAamp DNA Mini Kit and the Schleicher and Schuell IsoCode Stix DNA isolation device for isolating nucleic acid. Buffer, serum, and whole-blood samples were spiked with Bacillus anthracis Sterne vegetative cells and Yersinia pestis, while water was spiked with B. anthracis Sterne spores. Although minimal variations in limit of detection occurred among matrices, both the IsoCode Stix extraction method and the Qiagen procedure have comparable detection limits.

* Corresponding author. Mailing address: USAMRIID, Diagnostic Systems Division, 1425 Porter St., Fort Detrick, MD 21702-5011. Phone: (301) 619-6352. Fax: (301) 619-2492. E-mail: Melanie.Ulrich{at}amedd.army.mil.

Journal of Clinical Microbiology, October 2004, p. 4859-4862, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4859-4862.2004




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