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Journal of Clinical Microbiology, November 2004, p. 5053-5057, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5053-5057.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Mycobacterium africanum Genotyping Using Novel Spacer Oligonucleotides in the Direct Repeat Locus

Karine Brudey,1 M. Cristina Gutierrez,2 Véronique Vincent,2 Linda M. Parsons,3 Max Salfinger,3,4 Nalin Rastogi,1* and Christophe Sola1

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Pointe-à-Pitre, Guadeloupe,1 Centre National de Référence des Mycobactéries, Institut Pasteur, Paris, France,2 Clinical Mycobacteriology Laboratory, Wadsworth Center, New York State Department of Health,3 Department of Medicine, Albany Medical College, Albany, New York4

Received 4 December 2003/ Returned for modification 20 February 2004/ Accepted 23 June 2004

This study involves a first evaluation of 25 novel spacer oligonucleotides in addition to the 43 routine spacers for molecular characterization of a panel of 65 isolates of tubercle bacilli from different geographic origins that were initially classified as Mycobacterium africanum based on phenotypic characters. The 68-spacer format defined four additional patterns, and three groups were identified. The relatively homogeneous groups A1 and A2 included strains from West Africa, and A3-1 included strains from East Africa. The presence of deletion region RD9 confirmed the reclassification of the M. africanum subtype II spoligopattern within group A3-1 as Mycobacterium tuberculosis. These isolates may represent a diverging branch of M. tuberculosis in Africa. The use of new spacers also suggested an undergoing evolution of M. africanum subtype I in West Africa. Our results showed that the strain differentiation within the M. tuberculosis complex is improved by using novel spacers, and extensive studies using new-generation spoligotyping may be helpful to better understand the evolution of M. africanum.

* Corresponding author. Mailing address: Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Morne Jolivière, BP 484, 97165 Pointe-à-Pitre, Cedex, Guadeloupe. Phone: 590-(590)-893 881. Fax: 590-(590)-893 880. E-mail: nrastogi{at}pasteur-guadeloupe.fr.

Journal of Clinical Microbiology, November 2004, p. 5053-5057, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5053-5057.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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