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Journal of Clinical Microbiology, November 2004, p. 5327-5331, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5327-5331.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5' Nuclease Real-Time PCR Compared to a Modified Classical Approach

Guido Werner,* Birgit Strommenger, Ingo Klare, and Wolfgang Witte

Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany

Received 2 March 2004/ Returned for modification 27 May 2004/ Accepted 5 July 2004

A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.

* Corresponding author. Mailing address: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. Phone: 49 3943 679 210. Fax: 49 3943 679 207. E-mail: wernerg{at}rki.de.

Journal of Clinical Microbiology, November 2004, p. 5327-5331, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5327-5331.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Werner, G., Bartel, M., Wellinghausen, N., Essig, A., Klare, I., Witte, W., Poppert, S. (2007). Detection of Mutations Conferring Resistance to Linezolid in Enterococcus spp. by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 45: 3421-3423 [Abstract] [Full Text]  
  • Cieloszyk, K., Amodio-Groton, M., Saeed, M., Coyle, C. M (2007). Linezolid Resistance in Three Isolates of Coagulase-Negative Staphylococci. The Annals of Pharmacotherapy 41: 526-527 [Full Text]  
  • Seedat, J., Zick, G., Klare, I., Konstabel, C., Weiler, N., Sahly, H. (2006). Rapid Emergence of Resistance to Linezolid during Linezolid Therapy of an Enterococcus faecium Infection. Antimicrob. Agents Chemother. 50: 4217-4219 [Abstract] [Full Text]  


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