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Journal of Clinical Microbiology, November 2004, p. 5332-5333, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5332-5333.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Convenience of Serum for Visceral Leishmaniasis Diagnosis by PCR

Cristel Fissore,1* Pascal Delaunay,1 Bernard Ferrua,1 Eric Rosenthal,1 Pascal Del Giudice,1 Jean-Pierre Aufeuvre,2 Yves Le Fichoux,1 and Pierre Marty1

Equipe de Recherche sur les Leishmanioses, Faculté de Médecine, Université de Nice—Sophia-Antipolis and Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice, Nice, France,1 Centre de Transfusion Sanguine, Monaco2

Received 24 February 2004/ Returned for modification 27 February 2004/ Accepted 25 July 2004

In this retrospective study, the usefulness of a PCR performed on serum for primary diagnosis and monitoring of Mediterranean visceral leishmaniasis (MVL) was assessed. In the case of primary diagnosis of MVL, the serum PCR showed a sensitivity of 97% and a specificity of 95%, with positive and negative predictive values of 94 and 97%, respectively.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Hôpital de l'Archet, BP 3079, 06202 Nice Cedex 03, France. Phone: 33 (0)492036254. Fax: 33(0)492036258. E-mail: fissore.c{at}chu-nice.fr.

Journal of Clinical Microbiology, November 2004, p. 5332-5333, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5332-5333.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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