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Journal of Clinical Microbiology, December 2004, p. 5458-5461, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5458-5461.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Diagnosis of Schistosomiasis by Reagent Strip Test for Detection of Circulating Cathodic Antigen

G. J. van Dam,1* J. H. Wichers,2 T. M. Falcao Ferreira,1 D. Ghati,3 A. van Amerongen,2 and A. M. Deelder1

Department of Parasitology, Leiden University Medical Center, Leiden,1 Agrotechnology and Food Innovations, Wageningen University and Research Center, Wageningen, The Netherlands,2 National Institute for Medical Research, Mwanza Medical Research Center, Mwanza, Tanzania3

Received 6 February 2004/ Returned for modification 7 May 2004/ Accepted 24 June 2004

A newly developed reagent strip assay for the diagnosis of schistosomiasis based on parasite antigen detection in urine of infected individuals was evaluated. The test uses the principle of lateral flow through a nitrocellulose strip of the sample mixed with a colloidal carbon conjugate of a monoclonal antibody specific for Schistosoma circulating cathodic antigen (CCA). The strip assay to diagnose a group of highly infected schoolchildren in Mwanza, Tanzania, demonstrated a high sensitivity and association with the intensity of infection as measured both by egg counts, and by circulating anodic antigen and CCA levels determined by enzyme-linked immunosorbent assay. A specificity of ca. 90% was shown in a group of schistosome-negative schoolchildren from Tarime, Tanzania, an area where schistosomiasis is not endemic. The test is easy to perform and requires no technical equipment or special training. The stability of the strips and the conjugate in the dry format lasts for at least 3 months at ambient temperature in sealed packages, making it suitable for transport and use in areas where schistosomiasis is endemic. This assay can easily be developed to an end-user format.

* Corresponding author. Mailing address: Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands. Phone: 31-71-5265064. Fax: 31-71-5266907. E-mail: g.j.van_dam{at}lumc.nl.

Journal of Clinical Microbiology, December 2004, p. 5458-5461, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5458-5461.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Xia, X., Xu, Y., Zhao, X., Li, Q. (2009). Lateral Flow Immunoassay Using Europium Chelate-Loaded Silica Nanoparticles as Labels. Clin. Chem. 55: 179-182 [Abstract] [Full Text]  
  • Corstjens, P. L. A. M., van Lieshout, L., Zuiderwijk, M., Kornelis, D., Tanke, H. J., Deelder, A. M., van Dam, G. J. (2008). Up-Converting Phosphor Technology-Based Lateral Flow Assay for Detection of Schistosoma Circulating Anodic Antigen in Serum. J. Clin. Microbiol. 46: 171-176 [Abstract] [Full Text]  


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