This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shamputa, I. C.
Right arrow Articles by Portaels, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shamputa, I. C.
Right arrow Articles by Portaels, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2004, p. 5528-5536, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5528-5536.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotypic and Phenotypic Heterogeneity among Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients

Isdore Chola Shamputa,1* Leen Rigouts,1 Lovet Achale Eyongeta,1 Nabil Abdullah El Aila,1 Armand van Deun,1 Abdul Hamid Salim,2 Eve Willery,3 Camille Locht,3 Philip Supply,3 and Françoise Portaels1

Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium,1 Damien Foundation Bangladesh, Banani, Dhaka, Bangladesh,2 Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U629, Institut Pasteur de Lille, Lille, France3

Received 6 May 2004/ Returned for modification 16 June 2004/ Accepted 20 August 2004

Although the heterogeneity of Mycobacterium tuberculosis populations and the existence of mixed infections are now generally accepted, systematic studies on their relative importance are rare. In the present study, 10 individual colonies of each M. tuberculosis isolate (primary isolate) from 97 tuberculosis patients in a primarily human immunodeficiency virus-negative population were screened for heterogeneity and detectable mixed infections by spoligotyping, IS6110-based restriction fragment length polymorphism analysis, and mycobacterial interspersed repetitive unit-variable number of tandem repeat typing. The MICs of antituberculosis drugs for colonies with divergent fingerprints were determined. Infections with different bacterial subpopulations were detected in the samples from eight patients (8.2%), and the frequency of detectable mixed infections in the study population was estimated to be 2.1%. Genotypic variations were found to be independent of the drug susceptibilities, and the various molecular markers evolved independently in most cases. The predominant strains and the primary isolates always had concordant drug susceptibility and MIC testing results. These findings have implications on the interpretation of molecular epidemiology results for patient follow-up and in transmission studies.

* Corresponding author. Mailing address: Mycobacteriology Unit, Department of Microbiology, Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: 32 3 247 6336. Fax: 32 3 247 6333. E-mail: icshamputa{at}itg.be.

Journal of Clinical Microbiology, December 2004, p. 5528-5536, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5528-5536.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stavrum, R., Mphahlele, M., Ovreas, K., Muthivhi, T., Fourie, P. B., Weyer, K., Grewal, H. M. S. (2009). High Diversity of Mycobacterium tuberculosis Genotypes in South Africa and Preponderance of Mixed Infections among ST53 Isolates. J. Clin. Microbiol. 47: 1848-1856 [Abstract] [Full Text]  
  • Sunder, S., Lanotte, P., Godreuil, S., Martin, C., Boschiroli, M. L., Besnier, J. M. (2009). Human-to-Human Transmission of Tuberculosis Caused by Mycobacterium bovis in Immunocompetent Patients. J. Clin. Microbiol. 47: 1249-1251 [Abstract] [Full Text]  
  • Rahim, Z., Zaman, K., van der Zanden, A. G. M., Mollers, M. J., van Soolingen, D., Raqib, R., Zaman, K., Begum, V., Rigouts, L., Portaels, F., Rastogi, N., Sola, C. (2007). Assessment of Population Structure and Major Circulating Phylogeographical Clades of Mycobacterium tuberculosis Complex in Bangladesh Suggests a High Prevalence of a Specific Subclade of Ancient M. tuberculosis Genotypes. J. Clin. Microbiol. 45: 3791-3794 [Abstract] [Full Text]  
  • Traore, H., van Deun, A., Shamputa, I. C., Rigouts, L., Portaels, F. (2006). Direct Detection of Mycobacterium tuberculosis Complex DNA and Rifampin Resistance in Clinical Specimens from Tuberculosis Patients by Line Probe Assay. J. Clin. Microbiol. 44: 4384-4388 [Abstract] [Full Text]  
  • Gopaul, K. K., Brown, T. J., Gibson, A. L., Yates, M. D., Drobniewski, F. A. (2006). Progression Toward an Improved DNA Amplification-Based Typing Technique in the Study of Mycobacterium tuberculosis Epidemiology.. J. Clin. Microbiol. 44: 2492-2498 [Abstract] [Full Text]  
  • Shah, N. P., Singhal, A., Jain, A., Kumar, P., Uppal, S. S., Srivatsava, M. V. P., Prasad, H. K. (2006). Occurrence of Overlooked Zoonotic Tuberculosis: Detection of Mycobacterium bovis in Human Cerebrospinal Fluid. J. Clin. Microbiol. 44: 1352-1358 [Abstract] [Full Text]  
  • Garcia de Viedma, D., Alonso Rodriguez, N., Andres, S., Ruiz Serrano, M. J., Bouza, E. (2005). Characterization of Clonal Complexity in Tuberculosis by Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat Typing. J. Clin. Microbiol. 43: 5660-5664 [Abstract] [Full Text]  
  • van Deutekom, H., Supply, P., de Haas, P. E. W., Willery, E., Hoijng, S. P., Locht, C., Coutinho, R. A., van Soolingen, D. (2005). Molecular Typing of Mycobacterium tuberculosis by Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat Analysis, a More Accurate Method for Identifying Epidemiological Links between Patients with Tuberculosis. J. Clin. Microbiol. 43: 4473-4479 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»